Dietary polyphenol oleuropein and its metabolite hydroxytyrosol are moderate skin permeable elastase and collagenase inhibitors with synergistic cellular antioxidant effects in human skin fibroblasts. (19th May 2022)

• Record Type: Journal Article
• Authors: Li, Huifang; He, Hao; Liu, Chang; Akanji, Toyosi; Gutkowski, Justin; Li, Ruilian; Ma, Huiqun; Wan, Yinsheng; Wu, Panpan; Li, Dongli; Seeram, Navindra P.; Ma, Hang
• Is Part Of: International journal of food sciences and nutrition. Volume 73:Number 4(2022)
• Page Start: 460
• Page End: 470
• Publication Date: 2022-05-19
• Subjects: Oleuropein -- hydroxytyrosol -- elastase -- collagenase -- skin protection; Food -- Analysis -- Periodicals; Nutrition -- Periodicals; 641.3
• DOI: 10.1080/09637486.2021.1996542
• Languages: English
• ISSNs: 0963-7486

Abstract

Abstract: Oleuropein (OLE) and hydroxytyrosol (HT) are dietary polyphenols with skin beneficial effects but their effects on skin-ageing-related enzymes are not clear. Herein, we evaluated their inhibitory effects on elastase and collagenase. OLE and HT (62.5–1 000 μM) showed moderate anti-elastase and anti-collagenase effects (5.1–26.3%, 5.8–12.2% and 12.6–31.0%, 11.6–31.9% inhibition, respectively). Combinations of OLE and HT (1:1 ratio) exerted synergistic inhibitory effects on elastase, which were supported by their combination index (CI), kinetic assay and computational docking. Moreover, HT (100 μM) reduced hydrogen peroxide (H2 O2 )-induced cytotoxicity and reactive oxygen species (ROS) in human dermal fibroblast cells by 21.8 and 15.2%, respectively. In addition, combinations of OLE and HT (6.25/6.25–100/100 μM) exerted synergistic cytoprotective effects by reducing ROS levels by 7.6–37.3% with CIs of 0.17–0.44, respectively. The findings from this study support the cosmeceutical activities of OLE and HT but further research is warranted to evaluate their anti-skin-ageing effects using in vivo models.