An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake. Issue 2 (15th September 2015)

• Record Type: Journal Article
• Authors: He, Zhenyan; Yan, Huili; Chen, Yanshan; Shen, Hongling; Xu, Wenxiu; Zhang, Haiyan; Shi, Lei; Zhu, Yong‐Guan; Ma, Mi
• Is Part Of: New phytologist. Volume 209:Issue 2(2016)
• Page Start: 746
• Page End: 761
• Publication Date: 2015-09-15
• Subjects: aromatic/arginine (Ar/R) -- arsenic (As) -- arsenite (As(III)) -- aquaporin -- Pteris vittata -- PvTIP4;1 -- uptake; Botany -- Periodicals; 580
• DOI: 10.1111/nph.13637
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: The fern Pteris vittata is an arsenic hyperaccumulator. The genes involved in arsenite (As(III)) transport are not yet clear. Here, we describe the isolation and characterization of a new P. vittata aquaporin gene, PvTIP4;1, which may mediate As(III) uptake. PvTIP4;1 was identified from yeast functional complement cDNA library of P. vittata . Arsenic toxicity and accumulating activities of PvTIP4;1 were analyzed in Saccharomyces cerevisiae and Arabidopsis . Subcellular localization of PvTIP4;1–GFP fusion protein in P. vittata protoplast and callus was conducted. The tissue expression of PvTIP4;1 was investigated by quantitative real‐time PCR. Site‐directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied. Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. Transgenic Arabidopsis showed that PvTIP4;1 increases arsenic accumulation and induces arsenic sensitivity. Images and FM4‐64 staining suggest that PvTIP4;1 localizes to the plasma membrane in P. vittata cells. A tissue location study shows that PvTIP4;1 transcripts are mainly expressed in roots. Site‐directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site. PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin from P. vittata and the function and location results imply that PvTIP4;1 may be involved in As(III) uptake.