Standards for plant synthetic biology: a common syntax for exchange of DNA parts. Issue 1 (14th July 2015)

• Record Type: Journal Article
• Authors: Patron, Nicola J.; Orzaez, Diego; Marillonnet, Sylvestre; Warzecha, Heribert; Matthewman, Colette; Youles, Mark; Raitskin, Oleg; Leveau, Aymeric; Farré, Gemma; Rogers, Christian; Smith, Alison; Hibberd, Julian; Webb, Alex A. R.; Locke, James; Schornack, Sebastian; Ajioka, Jim; Baulcombe, David C.; Zipfel, Cyril; Kamoun, Sophien; Jones, Jonathan D. G.; Kuhn, Hannah; Robatzek, Silke; Van Esse, H. Peter; Sanders, Dale; Oldroyd, Giles; Martin, Cathie; Field, Rob; O'Connor, Sarah; Fox, Samantha; Wulff, Brande; Miller, Ben; Breakspear, Andy; Radhakrishnan, Guru; Delaux, Pierre‐Marc; Loqué, Dominique; Granell, Antonio; Tissier, Alain; Shih, Patrick; Brutnell, Thomas P.; Quick, W. Paul; Rischer, Heiko; Fraser, Paul D.; Aharoni, Asaph; Raines, Christine; South, Paul F.; Ané, Jean‐Michel; Hamberger, Björn R.; Langdale, Jane; Stougaard, Jens; Bouwmeester, Harro; Udvardi, Michael; Murray, James A. H.; Ntoukakis, Vardis; Schäfer, Patrick; Denby, Katherine; Edwards, Keith J.; Osbourn, Anne; Haseloff, Jim;
• Is Part Of: New phytologist. Volume 208:Issue 1(2015:Oct. 01)
• Page Start: 13
• Page End: 19
• Publication Date: 2015-07-14
• Subjects: cloning -- DNA assembly -- genetic syntax -- GoldenGate -- synthetic biology -- Type IIS restriction endonucleases; Botany -- Periodicals; 580
• DOI: 10.1111/nph.13532
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease‐mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.