Discovery of a subgenotype of human coronavirus NL63 associated with severe lower respiratory tract infection in China, 2018. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Wang, Yanqun; Li, Xin; Liu, Wenkuan; Gan, Mian; Zhang, Lu; Wang, Jin; Zhang, Zhaoyong; Zhu, Airu; Li, Fang; Sun, Jing; Zhang, Guoxian; Zhuang, Zhen; Luo, Jiaying; Chen, Dehui; Qiu, Shuyan; Zhang, Li; Xu, Duo; Mok, Chris Ka Pun; Zhang, Fuchun; Zhao, Jingxian; Zhou, Rong; Zhao, Jincun
• Is Part Of: Emerging microbes & infections. Volume 9:Issue 1(2020)
• Page Start: 246
• Page End: 255
• Publication Date: 2020-01-01
• Subjects: human coronavirus NL63 -- new subgenotype -- pneumonia -- whole-genome sequencing -- phylogenetic analysis -- viral entry; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1080/22221751.2020.1717999
• Languages: English
• ISSNs: 2222-1751

Abstract

ABSTRACT: Human coronavirus NL63 (HCoV-NL63) is primarily associated with common cold in children, elderly and immunocompromised individuals. Outbreaks caused by HCoV-NL63 are rare. Here we report a cluster of HCoV-NL63 cases with severe lower respiratory tract infection that arose in Guangzhou, China, in 2018. Twenty-three hospitalized children were confirmed to be HCoV-NL63 positive, and most of whom were hospitalized with severe pneumonia or acute bronchitis. Whole genomes of HCoV-NL63 were obtained using next-generation sequencing. Phylogenetic and single amino acid polymorphism analyses showed that this outbreak was associated with two subgenotypes (C3 and B) of HCoV-NL63. Half of patients were identified to be related to a new subgenotype C3. One unique amino acid mutation at I507 L in spike protein receptor binding domain (RBD) was detected, which segregated this subgenotype C3 from other known subgenotypes. Pseudotyped virus bearing the I507 L mutation in RBD showed enhanced entry into host cells as compared to the prototype virus. This study proved that HCoV-NL63 was undergoing continuous mutation and has the potential to cause severe lower respiratory disease in humans.