Laboratory management for SARS-CoV-2 detection: a user-friendly combination of the heat treatment approach and rt-Real-time PCR testing. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Mancini, Fabiola; Barbanti, Fabrizio; Scaturro, Maria; Errico, Giulia; Iacobino, Angelo; Bella, Antonino; Riccardo, Flavia; Marsili, Giulia; Stefanelli, Paola; Pezzotti, Patrizio; Rezza, Giovanni; Ciervo, Alessandra
• Is Part Of: Emerging microbes & infections. Volume 9:Issue 1(2020)
• Page Start: 1393
• Page End: 1396
• Publication Date: 2020-01-01
• Subjects: SARS-CoV-2 -- COVID 19 -- rt-Real time PCR -- molecular methods -- heat treatment -- nasopharyngeal samples; Medical microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.9041
• DOI: 10.1080/22221751.2020.1775500
• Languages: English
• ISSNs: 2222-1751

Abstract

ABSTRACT: The RNA purification is the gold standard for the detection of SARS-CoV-2 in swab samples, but it is dependent on the availability of chemical reagents. In this study, we evaluated the heat treatment method without RNA extraction as a reliable option to nucleic acid purification.