Anti-inflammatory effects of Capparis ecuadorica extract in phthalic-anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice. (1st January 2020)

• Record Type: Journal Article
• Authors: Song, Bo Ram; Lee, Su Jin; Kim, Ji Eun; Choi, Hyeon Jun; Bae, Su Ji; Choi, Yun Ju; Gong, Jeong Eun; Noh, Jin Kyung; Kim, Hye Sung; Kang, Hyun-Gu; Hong, Jin Tae; Hwang, Dae Youn
• Is Part Of: Pharmaceutical biology. Volume 58:Number 1(2020)
• Page Start: 1272
• Page End: 1285
• Publication Date: 2020-01-01
• Subjects: Inflammation -- iNOS -- COX-2 -- IgE -- luciferase signal; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.1080/13880209.2020.1856146
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context: The natural products derived from Capparis ecuadorica H.H. Iltis (Capparaceae) could have great potential for anti-inflammation since they inhibited the inflammatory response in lipopolysaccharide (LPS)‐stimulated RAW 264.7 cells. Object: This study investigated the anti-inflammatory effects and related mechanism of methanol extract of C. ecuadorica leaves (MCE) during atopic dermatitis (AD) responses. Materials and methods: Alterations in the phenotypical markers for AD, luciferase signal, iNOS‐mediated COX‐2 induction pathway, and inflammasome activation were analysed in non-Tg (n = 5) and 15% phthalic anhydride (PA) treated IL-4/Luc/CNS-1 transgenic (Tg) HR1 mice (n = 5 per group), subsequent to treatment with acetone-olive oil (AOO), vehicle (DMSO) and two dose MCE (20 and 40 mg/kg) three times a week for 4 weeks. Results: MCE treatment reduced the intracellular ROS level (48.2%), NO concentration (7.1 mmol/L) and inflammatory cytokine expressions (39.1%) in the LPS-stimulated RAW264.7 cells. A significant decrease was detected for ear thickness (16.9%), weight of lymph node (0.7 mg), IgE concentration (1.9 µg/mL), and epidermal thickness (31.8%) of the PA + MCE treated Tg mice. MCE treatment induced the decrease of luciferase signal derived from the IL-4 promoter and the recovery of the IL-4 downstream regulator cytokines. PA + MCE treated Tg mice showed decreasing infiltration of mast cells (42.5%), iNOS-mediated COX‐2 induction pathway, MAPK signalling pathway and inflammasome activation in the ear tissue. Conclusions: These findings provide the first evidence that MCE may have great potential to suppress chemical-induced skin inflammation through the suppression of IL-4 cytokine and the iNOS-mediated COX‐2 induction pathway, and activation of inflammasome.