Acute radiation syndrome-related gene expression in irradiated peripheral blood cell populations. (3rd April 2021)

• Record Type: Journal Article
• Authors: Ostheim, Patrick; Don Mallawaratchy, Alan; Müller, Thomas; Schüle, Simone; Hermann, Cornelius; Popp, Tanja; Eder, Stefan; Combs, Stephanie E.; Port, Matthias; Abend, Michael
• Is Part Of: International journal of radiation biology. Volume 97:Number 4(2021)
• Page Start: 474
• Page End: 484
• Publication Date: 2021-04-03
• Subjects: Gene signature -- peripheral blood -- blood cell populations -- radiation exposure -- biomarker -- HARS; Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.1080/09553002.2021.1876953
• Languages: English
• ISSNs: 0955-3002

Abstract

Abstract: Purpose: In a nuclear or radiological event, an early diagnostic tool is needed to distinguish the worried well from those individuals who may later develop life-threatenFing hematologic acute radiation syndrome. We examined the contribution of the peripheral blood's cell populations on radiation-induced gene expression (GE) changes. Materials and methods: EDTA-whole-blood from six healthy donors was X-irradiated with 0 and 4Gy and T-lymphocytes, B-lymphocytes, NK-cells and granulocytes were separated using immunomagnetic methods. GE were examined in cell populations and whole blood. Results: The cell populations contributed to the total RNA amount with a ratio of 11.6 for T-lymphocytes, 1.2 for B-cells, 1.2 for NK-cells, 1.0 for granulocytes. To estimate the contribution of GE per cell population, the baseline (0Gy) and the radiation-induced fold-change in GE relative to unexposed was considered for each gene. The T-lymphocytes (74.8%/80.5%) contributed predominantly to the radiation-induced up-regulation observed for FDXR/DDB2 and the B-lymphocytes (97.1%/83.8%) for down-regulated POU2AF1/WNT3 with a similar effect on whole blood gene expression measurements reflecting a corresponding order of magnitude. Conclusions: T- and B-lymphocytes contributed predominantly to the radiation-induced up-regulation of FDXR/DDB2 and down-regulation of POU2AF1/WNT3 . This study underlines the use of FDXR/DDB2 for biodosimetry purposes and POU2AF1/WNT3 for effect prediction of acute health effects.