The Glyphosate-Based Herbicide Roundup Does Not Elevate Genome-Wide Mutagenesis of Escherichia coli. Issue 10 (1st October 2017)

• Record Type: Journal Article
• Authors: Tincher, Clayton; Long, Hongan; Behringer, Megan; Walker, Noah; Lynch, Michael
• Is Part Of: G3. Volume 7:Issue 10(2017)
• Page Start: 3331
• Page End: 3335
• Publication Date: 2017-10-01
• Subjects: ecological dependence of mutations -- evolutionary genomics -- environmental mutagenesis -- mutagenicity test -- herbicide damage; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.117.300133
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of Escherichia coli after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.