An Eight-Parent Multiparent Advanced Generation Inter-Cross Population for Winter-Sown Wheat: Creation, Properties, and Validation. Issue 9 (1st September 2014)

• Record Type: Journal Article
• Authors: Mackay, Ian J; Bansept-Basler, Pauline; Barber, Toby; Bentley, Alison R; Cockram, James; Gosman, Nick; Greenland, Andy J; Horsnell, Richard; Howells, Rhian; O'Sullivan, Donal M; Rose, Gemma A; Howell, Phil J
• Is Part Of: G3. Volume 4:Issue 9(2014)
• Page Start: 1603
• Page End: 1610
• Publication Date: 2014-09-01
• Subjects: quantitative genetics -- genome-wide association scan -- single nucleotide polymorphism -- QTL mapping -- recombinant inbred line (RIL) -- high-density genotyping -- Multiparent Advanced Generation Inter-Cross (MAGIC) -- multiparental populations -- MPP; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.114.012963
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: MAGIC populations represent one of a new generation of crop genetic mapping resources combining high genetic recombination and diversity. We describe the creation and validation of an eight-parent MAGIC population consisting of 1091 F7 lines of winter-sown wheat ( Triticum aestivum L.). Analyses based on genotypes from a 90, 000-single nucleotide polymorphism (SNP) array find the population to be well-suited as a platform for fine-mapping quantitative trait loci (QTL) and gene isolation. Patterns of linkage disequilibrium (LD) show the population to be highly recombined; genetic marker diversity among the founders was 74% of that captured in a larger set of 64 wheat varieties, and 54% of SNPs segregating among the 64 lines also segregated among the eight founder lines. In contrast, a commonly used reference bi-parental population had only 54% of the diversity of the 64 varieties with 27% of SNPs segregating. We demonstrate the potential of this MAGIC resource by identifying a highly diagnostic marker for the morphological character "awn presence/absence" and independently validate it in an association-mapping panel. These analyses show this large, diverse, and highly recombined MAGIC population to be a powerful resource for the genetic dissection of target traits in wheat, and it is well-placed to efficiently exploit ongoing advances in phenomics and genomics. Genetic marker and trait data, together with instructions for access to seed, are available at http://www.niab.com/MAGIC/ .