A DNA Methylation Reader–Chaperone Regulator–Transcription Factor Complex Activates OsHKT1;5 Expression during Salinity Stress. Issue 11 (15th September 2020)

• Record Type: Journal Article
• Authors: Wang, Jie; Nan, Nan; Li, Ning; Liu, Yutong; Wang, Tian-Jing; Hwang, Inhwan; Liu, Bao; Xu, Zheng-Yi
• Is Part Of: The Plant Cell. Volume 32:Issue 11(2020)
• Page Start: 3535
• Page End: 3558
• Publication Date: 2020-09-15
• DOI: 10.1105/tpc.20.00301
• Languages: English
• ISSNs: 1040-4651

Abstract

Abstract : OsSUVH7, OsBAG4, and OsMYB106 form a DNA methylation reader–chaperone regulator–transcription factor complex on the promoter region of OsHKT1;5 to activate its expression during salinity stress. Abstract: Irrigated lands are increasingly salinized, which adversely affects agricultural productivity. To respond to high sodium (Na + ) concentrations, plants harbor multiple Na + transport systems. Rice ( Oryza sativa ) HIGH-AFFINITY POTASSIUM (K + ) TRANSPORTER1;5 (OsHKT1;5), a Na + -selective transporter, maintains K + /Na + homeostasis under salt stress. However, the mechanism regulating OsHKT1;5 expression remains unknown. Here, we present evidence that a protein complex consisting of rice BCL-2-ASSOCIATED ATHANOGENE4 (OsBAG4), OsMYB106, and OsSUVH7 regulates OsHKT1;5 expression in response to salt stress. We isolated a salt stress–sensitive mutant, osbag4-1, that showed significantly reduced OsHKT1;5 expression and reduced K + and elevated Na + levels in shoots. Using comparative interactomics, we isolate0d two OsBAG4-interacting proteins, OsMYB106 (a MYB transcription factor) and OsSUVH7 (a DNA methylation reader), that were crucial for OsHKT1;5 expression. OsMYB106 and OsSUVH7 bound to the MYB binding cis -element (MYBE) and the miniature inverted-repeat transposable element (MITE) upstream of the MYBE, respectively, in the OsHKT1;5 promoter. OsBAG4 functioned as a bridge between OsSUVH7 and OsMYB106 to facilitate OsMYB106 binding to the consensus MYBE in the OsHKT1;5 promoter, thereby activating the OsHKT1;5 expression. Elimination of the MITE or knockout of OsMYB106 or OsSUVH7 decreased OsHKT1;5 expression and increased salt sensitivity. Our findings reveal a transcriptional complex, consisting of a DNA methylation reader, a chaperone regulator, and a transcription factor, that collaboratively regulate OsHKT1;5 expression during salinity stress.