Reagent and Data Resources for Investigation of RNA Binding Protein Functions in Drosophila melanogaster Cultured Cells. Issue 9 (1st September 2015)

• Record Type: Journal Article
• Authors: Mohr, Stephanie E; Hu, Yanhui; Rudd, Kirstin; Buckner, Michael; Gilly, Quentin; Foster, Blake; Sierzputowska, Katarzyna; Comjean, Aram; Ye, Bing; Perrimon, Norbert
• Is Part Of: G3. Volume 5:Issue 9(2015)
• Page Start: 1919
• Page End: 1924
• Publication Date: 2015-09-01
• Subjects: Drosophila -- RNA binding protein -- RNAi -- data sharing -- high-throughput screen; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.115.019364
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: RNA binding proteins (RBPs) are involved in many cellular functions. To facilitate functional characterization of RBPs, we generated an RNA interference (RNAi) library for Drosophila cell-based screens comprising reagents targeting known or putative RBPs. To test the quality of the library and provide a baseline analysis of the effects of the RNAi reagents on viability, we screened the library using a total ATP assay and high-throughput imaging in Drosophila S2R+ cultured cells. The results are consistent with production of a high-quality library that will be useful for functional genomics studies using other assays. Altogether, we provide resources in the form of an initial curated list of Drosophila RBPs; an RNAi screening library we expect to be used with additional assays that address more specific biological questions; and total ATP and image data useful for comparison of those additional assay results with fundamental information such as effects of a given reagent in the library on cell viability. Importantly, we make the baseline data, including more than 200, 000 images, easily accessible online.