Phenotypes Associated with Second Chromosome P Element Insertions in Drosophila melanogaster. Issue 8 (1st August 2016)

• Record Type: Journal Article
• Authors: Kahsai, Lily; Millburn, Gillian H; Cook, Kevin R
• Is Part Of: G3. Volume 6:Issue 8(2016)
• Page Start: 2665
• Page End: 2670
• Publication Date: 2016-08-01
• Subjects: Drosophila melanogaster -- P element -- insertional mutagenesis -- complementation -- phenotypic characterization; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.116.030940
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: In Drosophila melanogaster, P element transposition has been a productive means of insertional mutagenesis. Thousands of genes have been tagged with natural and engineered P element constructs. Nevertheless, chromosomes carrying P element insertions tend to have high levels of background mutations from P elements inserting and excising during transposition. Consequently, the phenotypes seen when P element-bearing chromosomes are homozygous are often not attributable to the P insertions themselves. In this study, 178 strains in the Bloomington Drosophila Stock Center collection with P insertions on the second chromosome were complementation tested against molecularly defined chromosomal deletions and previously characterized single-gene mutations to determine if recessive lethality or sterility is associated with the P insertions rather than background mutations. This information should prove valuable to geneticists using these strains for experimental studies of gene function.