Site-Specific Nitrosoproteomic Identification of Endogenously S-Nitrosylated Proteins in Arabidopsis. Issue 4 (19th February 2015)

• Record Type: Journal Article
• Authors: Hu, Jiliang; Huang, Xiahe; Chen, Lichao; Sun, Xuwu; Lu, Congming; Zhang, Lixin; Wang, Yingchun; Zuo, Jianru
• Is Part Of: Plant physiology. Volume 167:Issue 4(2015)
• Page Start: 1731
• Page End: 1746
• Publication Date: 2015-02-19
• Subjects: Plant physiology -- Periodicals; Botany -- Periodicals; Periodicals; Electronic journals; 571.2
• DOI: 10.1104/pp.15.00026
• Languages: English
• ISSNs: 0032-0889

Abstract

Abstract : Proteome-wide identification of S-nitrosylated proteins in Arabidopsis by nitrosoproteomic analysis reveals the involvement of S-nitrosylation in diverse signaling pathways. Abstract: Nitric oxide (NO ) regulates multiple developmental events and stress responses in plants. A major biologically active species of NO is S -nitrosoglutathione (GSNO ), which is irreversibly degraded by GSNO reductase (GSNOR). The major physiological effect of NO is protein S -nitrosylation, a redox-based posttranslational modification mechanism by covalently linking an NO molecule to a cysteine thiol. However, little is known about the mechanisms of S -nitrosylation-regulated signaling, partly due to limited S -nitrosylated proteins being identified. In this study, we identified 1, 195 endogenously S -nitrosylated peptides in 926 proteins from the Arabidopsis ( Arabidopsis thaliana ) by a site-specific nitrosoproteomic approach, which, to date, is the largest data set of S -nitrosylated proteins among all organisms. Consensus sequence analysis of these peptides identified several motifs that contain acidic, but not basic, amino acid residues flanking the S -nitrosylated cysteine residues. These S -nitrosylated proteins are involved in a wide range of biological processes and are significantly enriched in chlorophyll metabolism, photosynthesis, carbohydrate metabolism, and stress responses. Consistently, the gsnor1-3 mutant shows the decreased chlorophyll content and altered photosynthetic properties, suggesting that S -nitrosylation is an important regulatory mechanism in these processes. These results have provided valuable resources and new clues to the studies on S -nitrosylation-regulated signaling in plants.