Major Improvements to the Heliconius melpomene Genome Assembly Used to Confirm 10 Chromosome Fusion Events in 6 Million Years of Butterfly Evolution. Issue 3 (1st March 2016)

• Record Type: Journal Article
• Authors: Davey, John W; Chouteau, Mathieu; Barker, Sarah L; Maroja, Luana; Baxter, Simon W; Simpson, Fraser; Merrill, Richard M; Joron, Mathieu; Mallet, James; Dasmahapatra, Kanchon K; Jiggins, Chris D
• Is Part Of: G3. Volume 6:Issue 3(2016)
• Page Start: 695
• Page End: 708
• Publication Date: 2016-03-01
• Subjects: Heliconius -- genome assembly -- linkage mapping -- chromosome fusions -- Eueides; Genetics -- Research -- Periodicals; Genomics -- Periodicals; Genetics; Genomics; Genes; Genetics -- Research; Genomics; Electronic journals; Periodical; Periodicals; Fulltext; Internet Resources; Periodicals; 572.8
• DOI: 10.1534/g3.115.023655
• Languages: English
• ISSNs: 2160-1836

Abstract

Abstract: The Heliconius butterflies are a widely studied adaptive radiation of 46 species spread across Central and South America, several of which are known to hybridize in the wild. Here, we present a substantially improved assembly of the Heliconius melpomene genome, developed using novel methods that should be applicable to improving other genome assemblies produced using short read sequencing. First, we whole-genome-sequenced a pedigree to produce a linkage map incorporating 99% of the genome. Second, we incorporated haplotype scaffolds extensively to produce a more complete haploid version of the draft genome. Third, we incorporated ∼20x coverage of Pacific Biosciences sequencing, and scaffolded the haploid genome using an assembly of this long-read sequence. These improvements result in a genome of 795 scaffolds, 275 Mb in length, with an N50 length of 2.1 Mb, an N50 number of 34, and with 99% of the genome placed, and 84% anchored on chromosomes. We use the new genome assembly to confirm that the Heliconius genome underwent 10 chromosome fusions since the split with its sister genus Eueides, over a period of about 6 million yr.