The cellulose synthase‐like F3 (CslF3) gene mediates cell wall polysaccharide synthesis and affects root growth and differentiation in barley. (4th May 2022)

• Record Type: Journal Article
• Authors: Lou, Haoyu; Tucker, Matthew R.; Shirley, Neil J.; Lahnstein, Jelle; Yang, Xiujuan; Ma, Chao; Schwerdt, Julian; Fusi, Riccardo; Burton, Rachel A.; Band, Leah R.; Bennett, Malcolm J.; Bulone, Vincent
• Is Part Of: Plant journal. Volume 110:Number 6(2022)
• Page Start: 1681
• Page End: 1699
• Publication Date: 2022-05-04
• Subjects: Oroot development -- cell wall -- cellulose synthase‐like gene -- glucoxylan -- Hordeum vulgare -- barley -- Arabidopsis; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.15764
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: The barley cellulose synthase‐like F ( CslF ) genes encode putative cell wall polysaccharide synthases. They are related to the cellulose synthase ( CesA ) genes involved in cellulose biosynthesis, and the CslD genes that influence root hair development. Although CslD genes are implicated in callose, mannan and cellulose biosynthesis, and are found in both monocots and eudicots, CslF genes are specific to the Poaceae. Recently the barley CslF3 ( HvCslF3 ) gene was shown to be involved in the synthesis of a novel (1, 4)‐β‐linked glucoxylan, but it remains unclear whether this gene contributes to plant growth and development. Here, expression profiling using qRT‐PCR and mRNA in situ hybridization revealed that HvCslF3 accumulates in the root elongation zone. Silencing HvCslF3 by RNAi was accompanied by slower root growth, linked with a shorter elongation zone and a significant reduction in root system size. Polymer profiling of the RNAi lines revealed a significant reduction in (1, 4)‐β‐linked glucoxylan levels. Remarkably, the heterologous expression of HvCslF3 in wild‐type (Col‐0) and root hair‐deficient Arabidopsis mutants ( csld3 and csld5 ) complemented the csld5 mutant phenotype, in addition to altering epidermal cell fate. Our results reveal a key role for HvCslF3 during barley root development and suggest that members of the CslD and CslF gene families have similar functions during root growth regulation. Significance Statement: Downregulation of the cellulose synthase‐like F3 barley ( Hordeum vulgare ) gene ( HvCslF3 ) negatively affects root elongation and leads to defects in meristem size and root hair elongation as well as altered (1, 4)‐β‐linked glucoxylan accumulation.