Nanoparticle‐Stabilized Emulsion Bioink for Digital Light Processing Based 3D Bioprinting of Porous Tissue Constructs. Issue 12 (4th March 2022)

• Record Type: Journal Article
• Authors: Tao, Jie; Zhu, Shunyao; Zhou, Nazi; Wang, Yu; Wan, Haoyuan; Zhang, Lin; Tang, Yaping; Pan, Yingzi; Yang, Yang; Zhang, Jianxiang; Liu, Rui
• Is Part Of: Advanced healthcare materials. Volume 11:Issue 12(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-03-04
• Subjects: digital light processing -- emulsion bioinks -- hydrogels -- macroporous structures -- tissue constructs; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.202102810
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract: A challenge for bioprinting tissue constructs is enabling the viability and functionality of encapsulated cells. Rationally designed bioink that can create appropriate biophysical cues shows great promise for overcoming such challenges. Here, a nanoparticle‐stabilized emulsion bioink for direct fabrication of porous tissue constructs by digital light processing based 3D bioprinting technology is introduced. The emulsion bioink is integrated by the mixture of aqueous dextran microdroplets and gelatin methacryloyl solution and is further rendered stable by β ‐lactoglobulin nanoparticles. After bioprinting, the printed tissue constructs create the macroporous structure via removal of dextran, thereby providing favorable biophysical cues to promote the viability, proliferation, and spreading of the encapsulated cells. Moreover, a trachea‐shaped construct containing chondrocytes is bioprinted and implanted in vivo. The results demonstrate that the generated macroporous construct is of benefit to cartilage tissue rebuilding. This work offers an advanced bioink for the fabrication of living tissue constructs by activating the cell behaviors and functions in situ and can lead to the development of 3D bioprinting. Abstract : A nanoparticle‐stabilized emulsion bioink is developed for direct fabrication of porous tissue constructs by digital light processing based 3D bioprinting technology. The printed construct hydrogels create the macroporous structure via removal of dextran phase, thereby providing favorable biophysical cues to promote the viability, proliferation, and spreading of the encapsulated cells.