Programmed Supramolecular Assemblies Using Orthogonal Pairs of Heterodimeric Coiled Coil Peptides. (5th May 2022)

• Record Type: Journal Article
• Authors: Jiang, Linhai; Zuo, Xiaobing; Li, Jianping; Traaseth, Nathaniel J.; Kirshenbaum, Kent
• Is Part Of: Angewandte Chemie. Volume 134:Number 27(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-05-05
• Subjects: α-Helices -- Coiled Coils -- Heterodimers -- Nanostructures -- Peptides -- Supramolecular Assemblies; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202201895
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Despite recent progress, it remains challenging to program biomacromolecules to assemble into discrete nanostructures with pre‐determined sizes and topologies. We report here a novel strategy to address this challenge. By using two orthogonal pairs of heterodimeric coiled coils as the building blocks, we constructed six discrete supramolecular assemblies, each composed of a prescribed number of coiled coil components. Within these assemblies, different coiled coils were connected via end‐to‐side covalent linkages strategically pre‐installed between the non‐complementary pairs. The overall topological features of two highly complex assemblies, a "barbell" and a "quadrilateral" form, were characterized experimentally and were in good agreement to the designs. This work expands the design paradigms for peptide‐based discrete supramolecular assemblies and will provide a route for de novo fabrication of functional protein materials. Abstract : A novel strategy for constructing peptide‐based supramolecular assemblies with pre‐determined oligomeric states and topologies is reported. By using four polypeptide chains that form two orthogonal heterodimeric coiled coils and pre‐installing covalent linkages between the non‐complementary pairs in strategic arrangements, several discrete supramolecular assemblies with pre‐determined molar masses and topologies were constructed.