One-pot bifunctionalization of silica nanoparticles conjugated with bioorthogonal linkers: application in dual-modal imaging. (25th May 2022)

• Record Type: Journal Article
• Authors: Lee, Jaewoon; Kim, Jeunghwan; Heo, Incheol; Kim, Su Jin; Jang, Sein; Lee, Ho-Young; Jang, Kwang-Suk; Yang, Chul-Su; Lee, Youngbok; Yoo, Won Cheol; Min, Sun-Joon
• Is Part Of: Biomaterials science. Volume 10:Number 13(2022)
• Page Start: 3540
• Page End: 3546
• Publication Date: 2022-05-25
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d2bm00258b
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Covalently conjugated bifunctional silica nanoparticles (SNPs) through one-pot bioorthogonal reactions in the application of in vivo dual imaging. Abstract : Covalent surface modification of silica nanoparticles (SNPs) offers great potential for the development of multimodal nanomaterials for biomedical applications. Herein, we report the synthesis of covalently conjugated bifunctional SNPs and their application to in vivo multimodal imaging. Bis(methallyl)silane 15 with cyclopropene and maleimide, designed as a stable bifunctional linker, was efficiently synthesized by traceless Staudiger ligation, and subsequently introduced onto the surface of monodispersed SNPs via Sc(OTf)3 -catalyzed siloxane formation. The bifunctional linker-grafted SNP 20 underwent both thiol-conjugated addition and tetrazine cycloaddition in one pot . Finally, positron emission tomography/computed tomography and fluorescence imaging study of dual functional SNP [ 125 I]28 labeled with NIR dye and 125 I isotope showed a prolonged circulation in mice, which is conducive to the systemic delivery of therapeutics.