Peptide functionalized DNA hydrogel enhances neuroblastoma cell growth and differentiation. Issue 24 (10th June 2022)

• Record Type: Journal Article
• Authors: Hivare, Pravin; Gangrade, Ankit; Swarup, Gitanjali; Bhavsar, Krishna; Singh, Ankur; Gupta, Ratnika; Thareja, Prachi; Gupta, Sharad; Bhatia, Dhiraj
• Is Part Of: Nanoscale. Volume 14:Issue 24(2022)
• Page Start: 8611
• Page End: 8620
• Publication Date: 2022-06-10
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d1nr07187d
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Designing programmable biomaterials that could act as extracellular matrices and permit functionalization is a current need for tissue engineering advancement. Abstract : Designing programmable biomaterials that could act as extracellular matrices and permit functionalization is a current need for tissue engineering advancement. DNA based hydrogels are gaining significant attention owing to their self-assembling properties, biocompatibility, chemical robustness and low batch to batch variability. The real potential of DNA hydrogels in the biomedical domain remains to be explored. In this work, a DNA hydrogel was coated on a glass surface and coupled to a synthetic IKVAV peptide by a chemical crosslinker. We observe enhanced neuronal differentiation, prolonged neurite length, dynamic movement of microtubules and cytoskeleton, and altered endocytic mechanisms in neuroblastoma-based stem cells for the peptide modified DNA hydrogel compared to the unmodified DNA hydrogel and controls. We anticipate that a peptide-modified DNA hydrogel could emerge as a promising scaffold coating material to develop nerve tissue conduits in the future for application in neuroscience and neuroregeneration.