Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells. Issue 24 (8th June 2022)

• Record Type: Journal Article
• Authors: Savchak, Oksana K.; Wang, Nanying; Ramos-Docampo, Miguel A.; de Dios Andres, Paula; Sebastião, Ana M.; Ribeiro, Filipa F.; Armada-Moreira, Adam; Städler, Brigitte; Vaz, Sandra H.
• Is Part Of: Journal of materials chemistry. Volume 10:Issue 24(2022)
• Page Start: 4672
• Page End: 4683
• Publication Date: 2022-06-08
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb00393g
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : Reactors equipped with manganese dioxide nanosheets can protect mammalian cells from reactive oxygen species (ROS), such as hydrogen peroxide. Especially, small reactors that can self-navigate in solution showed an enhanced removal of ROS. Abstract : Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H2 O2 ) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO2 -NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO2 -NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H2 O2 . Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO2 -NSs showed directed locomotion in cell media with top speeds up to 50 μm s −1 when exposed to 300 mM H2 O2 as a fuel, while reactors homogeneously coated with MnO2 -NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H2 O2 from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.