Mechanism of Phanerochaete chrysosporium-mediated tetracycline hydrochloride removal promoted by low-dose silver nanoparticles. Issue 3 (June 2022)

• Record Type: Journal Article
• Authors: Liu, Rui; Guo, Zhi; Li, Jie; Wu, Feiyan; Cui, Kangping; Cheng, Peng; Chen, Yihan; Ding, Yan; Cui, Minshu; Wu, Zhangzhen
• Is Part Of: Journal of environmental chemical engineering. Volume 10:Issue 3(2022)
• Publication Date: 2022-06
• Subjects: Tetracycline hydrochloride -- Activity -- O2 flux -- Cytochrome P450 -- Extracellular enzymes; Chemical engineering -- Environmental aspects -- Periodicals; Environmental engineering -- Periodicals; Chemical engineering -- Environmental aspects; Environmental engineering; Periodicals; 660.0286
• DOI: 10.1016/j.jece.2022.107474
• Languages: English
• ISSNs: 2213-2929

Abstract

Abstract: The influence of silver nanoparticles (AgNPs) on environmental microorganisms has been extensively reported, however, there is currently limited understanding of effects of stress-induced by AgNPs on other pollutant degradation. In this study, Phanerochaete chrysosporium was stimulated by different concentrations of AgNPs to explore its effect on P. chrysosporium -mediated degradation of tetracycline hydrochloride (TCH). The results indicate that treatment with AgNPs at low concentration resulted in a considerable increase in TCH degradation, TCH degradation reached a maximum of 97% with 0.1 mg L −1 AgNPs treated after 3 d, and the viability of P. chrysosporium increased 1.48 times compared with the control group. The largest O2 influx of − 17 pmol cm −2 s −1 under 0.1 mg L −1 AgNPs treatment reveal the enhancement of P. chrysosporium respiration. In addition, low concentrations of AgNPs could induce the overexpression of cytochrome P450 and extracellular degradation enzymes MnP-related genes in stimulated cells, which further induce the production of extracellular degrading enzymes (51.0 U L −1 MnP and 23.7 U L −1 LiP) to accelerate the degradation of TCH. Together, our combined results uncovered the enhanced degradation mechanisms of P. chrysosporium induced by which AgNPs under sublethal dosage. Graphical Abstract: ga1 Highlights: Low dose AgNPs (0.01–0.1 mg L −1 ) promote the biodegradation of TCH. P. chrysosporium activity was increased by 1.48 times under 0.1 mg L −1 AgNPs treatment. Low-level AgNPs stress significantly enhanced the absorption rate of O2 . Increased expression of cytochrome P450 and extracellular enzyme promoted the degradation of antibiotics.