Layers 3 and 4 Neurons of the Bilateral Whisker-Barrel Cortex. (1st July 2022)

• Record Type: Journal Article
• Authors: Tsytsarev, Vassiliy; Kwon, Sung E.; Plachez, Celine; Zhao, Shuxin; O'Connor, Daniel H.; Erzurumlu, Reha S.
• Is Part Of: Neuroscience. Volume 494(2022)
• Page Start: 140
• Page End: 151
• Publication Date: 2022-07-01
• Subjects: CO cytochrome oxidase -- HGPPS horizontal gaze palsy with progressive scoliosis -- IOS intrinsic optical signal -- PrV principal nucleus -- ROIs regions of interest -- VPM ventroposteromedial; conditional Robo3 knockout -- dendritic complexity -- Ca2+-fluorescence protein -- In vivo 2-photon imaging -- somatosensory cortex -- whiskers; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2022.05.018
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: Bilateral whisker representations in the somatosensory thalamus and cortex. Smaller barrels and more focalized, simpler dendritic fields. Bilateral whisker stimulation activates neighboring groups of layer 3 neurons. Abstract: In Robo3 R3-5 cKO mouse brain, rhombomere 3-derived trigeminal principal nucleus (PrV) neurons project bilaterally to the somatosensory thalamus. As a consequence, whisker-specific neural modules (barreloids and barrels) representing whiskers on both sides of the face develop in the sensory thalamus and the primary somatosensory cortex. We examined the morphological complexity of layer 4 barrel cells, their postsynaptic partners in layer 3, and functional specificity of layer 3 pyramidal cells. Layer 4 spiny stellate cells form much smaller barrels and their dendritic fields are more focalized and less complex compared to controls, while layer 3 pyramidal cells did not show notable differences. Using in vivo 2-photon imaging of a genetically encoded fluorescent [Ca 2+ ] sensor, we visualized neural activity in the normal and Robo3 R3-5 cKO barrel cortex in response to ipsi- and contralateral single whisker stimulation. Layer 3 neurons in control animals responded only to their contralateral whiskers, while in the mutant cortex layer 3 pyramidal neurons showed both ipsi- and contralateral whisker responses. These results indicate that bilateral whisker map inputs stimulate different but neighboring groups of layer 3 neurons which normally relay contralateral whisker-specific information to other cortical areas.