CircRNA-ABCB10 promotes gastric cancer progression by sponging miR-1915–3p to upregulate RaC1. Issue 7 (July 2022)

• Record Type: Journal Article
• Authors: Liu, Junhui; Qiu, Guanglin; Wang, Haijiang; Li, Na; Liao, Xinhua
• Is Part Of: Digestive and liver disease. Volume 54:Issue 7(2022)
• Page Start: 896
• Page End: 904
• Publication Date: 2022-07
• Subjects: Circ-ABCB10 -- Gastric cancer -- miR-1915–3p -- Rac1 -- Tumorigenesis; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; 616.33005
• DOI: 10.1016/j.dld.2021.12.001
• Languages: English
• ISSNs: 1590-8658

Abstract

Abstract: Background: Gastric cancer (GC) is a common malignant tumor of the digestive system. Increasing reports have demonstrated the crucial roles of circRNAs in tumorigenesis and progression of GC. Methods: The relative expression of circ-ABCB10 in GC tissues and cell lines was detected by qRT-PCR. A series of in vitro assays and a xenograft model in vivo were applied to explore the function of circ-ABCB10 in GC cells. Results: Circ-ABCB10 expression was upregulated in GC tissues and cell lines and positively correlated with poor survival of GC patients. Circ-ABCB10 downregulation decreased cell viability, inhibited cell growth, invasion, and migration, while promoted cell apoptosis of GC cell lines SGC-7901 and MKN-48. Circ-ABCB10 could upregulate Rac1 expression by directly sponging miR-1915–3p. Rescue experiments revealed that miR-1915–3p inhibitor obviously reversed the inhibitory effect of si-circ-ABCB10, and Rac1 overexpression obviously reversed the inhibitory effect of miR-1915–3p mimics on cell growth, invasion, migration, apoptosis, and cell cycle progression. Moreover, si-circ-ABCB10 effectively inhibited tumor growth in a xenograft model. Conclusions: Our study revealed that circ-ABCB10 promoted GC progression via targeting the miR-1915–3p/Rac1 axis, and circ-ABCB10 might be a potential target for GC diagnosis and treatment.