Enhanced photoactivity of CdS nanorods by MXene and ZnSnO3: Application in photoelectrochemical biosensor for the effect of environmental pollutants on DNA hydroxymethylation in wheat tissues. (June 2022)

• Record Type: Journal Article
• Authors: Zheng, Yulin; Zhou, Yunlei; Cui, Xiaoting; Yin, Huanshun; Ai, Shiyun
• Is Part Of: Materials today chemistry. Volume 24(2022)
• Publication Date: 2022-06
• Subjects: ZnSnO3 nanosphere -- 5-Hydroxymethylcytosine -- Photoelectrochemistry -- Eecotoxicity -- Perfluorinated compound -- Heavy metal; Chemistry -- Periodicals; Materials -- Research -- Periodicals; Materials science -- Periodicals; Chemistry; Materials -- Research; Electronic journals; Periodicals; 660.282
• DOI: 10.1016/j.mtchem.2022.100878
• Languages: English
• ISSNs: 2468-5194

Abstract

Abstract: The photoactivity of CdS nanorods was greatly improved by amino functionalized accordion-like MXene and spherical ZnSnO3 . MXene possesses good electron transfer capability and ZnSnO3 presents matched energy band with CdS, which deeply accelerate the electron transfer and prevent the recombination of photogenerated electron-hole pair, leading to a strong photoelectrochemical (PEC) response. Taking the merit of the improved photoactivity of CdS nanorods, a novel PEC biosensor was constructed for DNA hydromethylation detection based on immune recognition of target molecule, where 5-hydroxymethyl-2′-deoxycytidine triphosphate (5hmdCTP) was employed as detect target, CdS/MXene was used as photoactive material, and ZnSnO3 was adopted as signal amplification unit. Under enzymatic covalent reaction of –CH2 OH of 5hmdCTP with –NH2 of MXene, 5hmdCTP was specifically recognized and captured. Then, taking advantages of the covalent reaction between phosphate group of 5hmdCTP and ZnSnO3, the signal amplification unit was captured. Under the optimum conditions, this PEC biosensor presents wide linear range of 0.008–100 nM and low detection limit of 4.21 pM (3σ). The applicability of the developed method was evaluated by investigating the effect of Cd 2+ and perfluorohexane compound pollutant on 5-hydroxymethylcytosine content in the genomic DNA of the roots and leaves of wheat seedlings. Graphical abstract: Image 1 Highlights: The aminated MXene greatly improves the photoelectric activity of CdS. DNA hydroxymethylation was specifically recognized and detected by a photoelectrochemical biosensor. The photocurrent response is enhanced by the band matching of CdS/MXene-NH2 /ZnSnO3 . The effects of antibiotics and heavy metals on the expression of 5hmdC were investigated.