Bactericidal effect of laser synthesized silver nanoparticle-impregnated cellulose paper against E. Coli. (October 2022)

• Record Type: Journal Article
• Authors: Ahsan, A.; Kamran, H.; Malik, A.; Saeed, M.M.; Awan, H.M.; Qayyum, H.
• Is Part Of: Optics & laser technology. Volume 154(2022)
• Publication Date: 2022-10
• Subjects: Optics -- Periodicals; Lasers -- Periodicals; Electronic journals; 621.366
• DOI: 10.1016/j.optlastec.2022.108305
• Languages: English
• ISSNs: 0030-3992

Abstract

Highlights: Bare silver nanoparticles were synthesized using pulsed laser ablation in liquid. Antimicrobial activity of laser synthesized nanoparticles was tested against ampicillin-resistant Escherichia coli . Silver nanoparticles embedded filter papers were used for point of use POU water disinfection. The anti-bacterial activity of the filter paper increases both with increasing amounts of Silver nanoparticles and immersion time. Absolute bactericidal activity can be achieved within 15 min on incubation with contaminated water. Multiple use of Silver nanoparticles embedded filter papers did not show any considerable decline in their bactericidal efficacy. Abstract: The search for affordable, safe, and effective water disinfection method is being pursued for several decades. In this study, we evaluated the efficacy of silver nanoparticles embedded filter paper for the removal of antibiotic-resistant bacteria from water. Pulsed laser ablation of a pure silver target in distilled liquid is used to synthesize bare AgNPs with a mean diameter of 25 nm. The concentration of 20 µg/ml of AgNPs gave a decent 18 mm zone of inhibition against ampicillin-resistant E. coli on Lysogeny Broth (LB) agar plates. When embedded onto filter paper, 26 µg of Ag NPs demonstrated an absolute bactericidal activity within 15 min of incubation with antibiotic-resistant E. coli containing water. Furthermore, the absence of bacteria from the filtrate suggests the removal of bacteria from the water. Reusing AgNP-embedded filter paper at least three times showed no substantial decline in its bactericidal activity and exhibited almost 100% reduction in bacterial growth.