Enhanced removal of PAHs by Peniophora incarnata and ascertainment of its novel ligninolytic enzyme genes. (1st December 2015)

• Record Type: Journal Article
• Authors: Lee, Hwanhwi; Jang, Yeongseon; Lee, Young Min; Lee, Hanbyul; Kim, Gyu-Hyeok; Kim, Jae-Jin
• Is Part Of: Journal of environmental management. Volume 164(2015)
• Page Start: 10
• Page End: 18
• Publication Date: 2015-12-01
• Subjects: Anthracene -- Ligninolytic enzymes -- PAHs -- Peniophora incarnata -- KUC8836 -- Pyrene -- Tween 80; Environmental policy -- Periodicals; Environmental management -- Periodicals; Environment -- Periodicals; Ecology -- Periodicals; 363.705
• DOI: 10.1016/j.jenvman.2015.08.036
• Languages: English
• ISSNs: 0301-4797

Abstract

Abstract: The hazardous effects of the PAHs should be managed by removal using white rot fungal ligninolytic enzymes. The white rot fungus Peniophora incarnata KUC8836 was stimulated to produce ligninolytic enzymes in a liquid medium by the addition of four substances: 0.5 g L −1 Tween 80, 70 mg L −1 CuSO4 ·5H2 O, 10 mg L −1 MnSO4 ·H2 O, and 0.3 g L −1 veratryl alcohol. The experiments were carried out in two different media: basal salt and 2% malt extract (ME) liquid medium. Under the experimental conditions, both laccase and manganese-dependent peroxidase (MnP) demonstrated with the highest activities in 2% ME liquid medium following the addition of Tween 80. The biodegradation of anthracene and pyrene was significantly enhanced by the induced ligninolytic enzymes when Tween 80 was added. Tween 80 is a viable co-substrate for P. incarnata, as it enhances the ability of P. incarnata to manage effective biodegradation of PAHs. Most of all, the novel laccase and MnP genes ascertained in this study, showed that the genes were involved in the production of ligninolytic enzymes from P. incarnata KUC8836 . Highlights: Ligninolytic enzymes were induced by tween 80. Biodegradation rates of PAHs were enhanced by induced ligninolytic enzymes. Enhanced biodegradation rates were verified by kinetics. Two novel genes involved in laccase and MnP imply the degradation of PAHs.