High-Energy Electron Scattering in Thick Samples Evaluated by Bright-Field Transmission Electron Microscopy, Energy-Filtering Transmission Electron Microscopy, and Electron Tomography. (June 2022)

• Record Type: Journal Article
• Authors: Hayashida, Misa; Malac, Marek
• Is Part Of: Microscopy and microanalysis. Volume 28:Number 3(2022)
• Page Start: 659
• Page End: 671
• Publication Date: 2022-06
• Subjects: Beer–Lambert law -- bright-field TEM -- electron energy-loss spectroscopy (EELS) -- energy-filtering TEM -- multiple scattering -- thick sample -- thickness measurement -- transmission electron microscopy (TEM); Microscopy -- Periodicals; Microchemistry -- Periodicals; 502.82
• DOI: 10.1017/S1431927622000472
• Languages: English
• ISSNs: 1431-9276

Abstract

Abstract: Abstract : Energy-filtering transmission electron microscopy (TEM) and bright-field TEM can be used to extract local sample thickness $t$ and to generate two-dimensional sample thickness maps. Electron tomography can be used to accurately verify the local $t$ . The relations of log-ratio of zero-loss filtered energy-filtering TEM beam intensity ( $I_{{\rm ZLP}}$ ) and unfiltered beam intensity ( $I_{\rm u}$ ) versus sample thickness $t$ were measured for five values of collection angle in a microscope equipped with an energy filter. Furthermore, log-ratio of the incident (primary) beam intensity ( $I_{\rm p}$ ) and the transmitted beam $I_{{\rm tr}}$ versus $t$ in bright-field TEM was measured utilizing a camera before the energy filter. The measurements were performed on a multilayer sample containing eight materials and thickness $t$ up to 800 nm. Local thickness $t$ was verified by electron tomography. The following results are reported: The maximum thickness $t_{{\rm max}}$ yielding a linear relation of log-ratio, $\ln ( {I_{\rm u}}/{I_{{\rm ZLP}}})$ and $\ln ( {I_{\rm p}}/{I_{{\rm tr}}} )$, versus $t$ . Inelastic mean free path ( $\lambda _{{\rm in}}$ ) for five values of collection angle. Total mean free path ( $\lambda _{{\rm total}}$ ) of electrons excluded by an angle-limiting aperture. $\lambda _{{\rm in}}$ and $\lambda _{{\rm total}}$ are evaluated for the eight materials with atomic number from $\approx$ 10 to 79. The results can be utilized as a guide for upper limit of $t$ evaluation in energy-filtering TEM and bright-field TEM and for optimizing electron tomography experiments.