Tumor‐derived immunoglobulin like transcript 5 induces suppressive immunocyte infiltration in colorectal cancer. Issue 6 (28th April 2022)

• Record Type: Journal Article
• Authors: Shi, Wenjing; Zhang, Fang; Chen, Xiaozheng; Wang, Shuyun; Zhang, Haiqin; Yang, Zijiang; Wang, Guiying; Zheng, Yan; Han, Yali; Sun, Yuping; Gao, Aiqin
• Is Part Of: Cancer science. Volume 113:Issue 6(2022)
• Page Start: 1939
• Page End: 1954
• Publication Date: 2022-04-28
• Subjects: colorectal cancer -- immunoglobulin‐like transcript 5 -- immunosuppression -- M2‐like tumor‐associated macrophages -- T cell subsets; Cancer -- Periodicals; Neoplasms -- Periodicals; Research -- Periodicals; Electronic journals; 616.994005
• DOI: 10.1111/cas.15360
• Languages: English
• ISSNs: 1347-9032

Abstract

Abstract: Infiltration of immunosuppressive cells in the tumor microenvironment (TME) induced colorectal cancer (CRC) progression and its resistance to immunotherapy. Identification of tumor‐specific factors to modulate inhibitory immunocyte infiltration would provide alternative and novel targets for CRC immunotherapy. Immunoglobulin‐like transcript (ILT) 5 is a negative regulator of myeloid cell activation. However, its expression and functional role in solid tumors is still unknown. Using human CRC tissues and cell lines, we found that ILT5 was highly expressed in CRC cells compared with normal colorectal epithelial cells. Enriched ILT5 in tumor cells was correlated with advanced tumor stages and poor patient survival. Our subsequent in vitro and in vivo studies revealed that tumor‐derived ILT5 inhibited the infiltration of T cells, especially that of CD8 + T cells in the TME, creating suppressive T‐cell contexture. Furthermore, ILT5 directed M2‐like polarization of tumor‐associated macrophages (TAMs). Inhibition of tumor‐derived ILT5 restored the immunosuppressive T‐cell and TAM contexture, and restricted CRC progression. Our findings identified ILT5 expression in solid tumor cells for the first time and raised ILT5 as a potential immunotarget and prognostic predictor in CRC. Abstract : Immunoglobulin‐like transcript 5 (ILT5) is enriched in colorectal cancer (CRC) cells, functioning as a negative prognostic biomarker. Enriched ILT5 in CRC cells inhibited the infiltration of T cells, especially that of CD8 + T cells in the tumor microenvironment (TME) and directed M2‐like polarization of tumor‐associated macrophages. Inhibition of tumor‐derived ILT5 restored the immunosuppressive TME and restricted CRC progression.