PHOSPHATE exporter XPR1/SLC53A1 is required for the tumorigenicity of epithelial ovarian cancer. Issue 6 (24th April 2022)

• Record Type: Journal Article
• Authors: Akasu‐Nagayoshi, Yoko; Hayashi, Tomoatsu; Kawabata, Ayako; Shimizu, Naomi; Yamada, Ai; Yokota, Naoko; Nakato, Ryuichiro; Shirahige, Katsuhiko; Okamoto, Aikou; Akiyama, Tetsu
• Is Part Of: Cancer science. Volume 113:Issue 6(2022)
• Page Start: 2034
• Page End: 2043
• Publication Date: 2022-04-24
• Subjects: apoptosis -- ovarian cancer -- phosphate transporter -- proliferation -- tumorigenicity -- XPR1; Cancer -- Periodicals; Neoplasms -- Periodicals; Research -- Periodicals; Electronic journals; 616.994005
• DOI: 10.1111/cas.15358
• Languages: English
• ISSNs: 1347-9032

Abstract

Abstract: Ovarian cancer is the fifth most common cause of cancer‐related death in women. Ovarian clear cell carcinoma (OCCC) is a chemotherapy‐resistant epithelial ovarian cancer with poor prognosis. As a basis for the development of therapeutic agents that could improve the prognosis of OCCC, we performed a screen for proteins critical for the tumorigenicity of OCCC using the CRISPR/Cas9 system. Here we show that knockdown of the phosphate exporter XPR1/SLC53A1 induces the growth arrest and apoptosis of OCCC cells in vitro. Moreover, we show that knockdown of XPR1/SLC53A1 inhibits the proliferation of OCCC cells xenografted into immunocompromised mice. These results suggest that XPR1/SLC53A1 plays a critical role in the tumorigenesis of OCCC cells. We speculate that XPR1/SLC53A1 might be a promising molecular target for the therapeutic treatment of OCCC. Abstract : We attempted to identify novel molecular targets critical for the proliferation and tumorigenicity of ovarian clear cell carcinoma (OCCC) using the CRISPR/Cas9 system. In this study, we have found that the phosphate exporter XPR1/SLC53A1 plays an important role in the proliferation and tumorigenicity of OCCC. Our results suggest that XPR1/SLC53A1 might be a promising molecular target for the therapeutic treatment of OCCC.