MiniTurbo‐based interactomics of two plasma membrane‐localized SNARE proteins in Marchantia polymorpha. Issue 2 (30th April 2022)

• Record Type: Journal Article
• Authors: Melkonian, Katharina; Stolze, Sara Christina; Harzen, Anne; Nakagami, Hirofumi
• Is Part Of: New phytologist. Volume 235:Issue 2(2022)
• Page Start: 786
• Page End: 800
• Publication Date: 2022-04-30
• Subjects: interactomics -- Marchantia polymorpha -- membrane‐trafficking -- miniTurbo -- proximity labelling -- SNARE protein; Botany -- Periodicals; 580
• DOI: 10.1111/nph.18151
• Languages: English
• ISSNs: 0028-646X

Abstract

Summary: Marchantia polymorpha is a model liverwort and its overall low genetic redundancy is advantageous for dissecting complex pathways. Proximity‐dependent in vivo biotin‐labelling methods have emerged as powerful interactomics tools in recent years. However, interactomics studies applying proximity labelling are currently limited to angiosperm species in plants. Here, we established and evaluated a miniTurbo‐based interactomics method in M. polymorpha using MpSYP12A and MpSYP13B, two plasma membrane‐localized SNARE proteins, as baits. We show that our method yields a manifold of potential interactors of MpSYP12A and MpSYP13B compared to a coimmunoprecipitation approach. Our method could capture specific candidates for each SNARE. We conclude that a miniTurbo‐based method is a feasible tool for interactomics in M . polymorpha and potentially applicable to other model bryophytes. Our interactome dataset on MpSYP12A and MpSYP13B will be a useful resource to elucidate the evolution of SNARE functions.