Omentin-1 alleviate interleukin-1β(IL-1β)-induced nucleus pulposus cells senescence. Issue 5 (2nd May 2022)

• Record Type: Journal Article
• Authors: Huang, Xin; Chen, Changhong; Chen, Yaofei; Xu, Jun; Liu, Lin
• Is Part Of: Bioengineered. Volume 13:Issue 5(2022)
• Page Start: 13849
• Page End: 13859
• Publication Date: 2022-05-02
• Subjects: Intervertebral disc degeneration -- Omentin-1 -- HNPCs -- senescence -- SIRT1; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2022.2084495
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: One of the main causes of low back pain (LBP) and degenerative musculoskeletal disorders is intervertebral disc degeneration (IVDD). Inflammation-associated senescence of Human nucleus pulposus cells (HNPCs) plays an essential function in the disease progression of IVDD. Omentin-1 is an adipokine that has been recently reported to have anti-inflammatory potential. In our research, IL-1β was used to simulate the inflammatory environment in the IVDD. We investigated in vitro the effects of Omentin-1 on HNPCs, including the components of senescence, cell cycle and extracellular matrix (ECM) synthesis. The results showed that the addition of Omentin-1 improved IL-1β-induced senescence in HNPCs. G1 phase cell cycle arrest and reduced ECM synthesis in HNPCs. Furthermore, we demonstrated that the effect of Omentin-1 in reducing senescence of HNPCs is dependent on SIRT1. These findings suggest that Omentin-1 plays an important function in protecting HNPCs against senescence and has the potential for IVDD gene target therapy. Graphical abtract: uf0001