Determination of glucosamine and galactosamine in food by liquid chromatography with pre-column derivatization. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Mu, Li; Xu, Ying; Li, Gang; Dai, Shuo; Tong, Qi; Liu, Bin
• Is Part Of: Food and agricultural immunology. Volume 33:Issue 1(2022)
• Page Start: 419
• Page End: 437
• Publication Date: 2022-12-31
• Subjects: High-performance liquid chromatography (HPLC) -- glucosamine (GlcN) -- galactosamine (GalN) -- 9-fluorenylmethoxycarbonyl chloride (FMOC-Cl); Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2022.2085673
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: A pre-column derivatization liquid chromatographic method was developed for the simultaneous determination of both glucosamine and galactosamine in selected food samples. The derivatization of glucosamine and galactosamine with 9-fluorenylmethoxycarbonyl chloride was accomplished under alkaline conditions and UV irradiation. When the detection wavelength was 265 nm and acetonitrile-0.1% phosphoric acid was the mobile phase at a flow rate of 1.0 mL/min, the linear range of ultraviolet intensity of glucosamine and galactosamine was 10–100 μg/mL, correlation coefficient of glucosamine was 0.9994 and correlation coefficient of galactosamine was 0.9993. The standard recovery of three kinds of food was in the range of 94.09–114.5%, the relative standard deviation (RSD) was 0.38–5.29%, the detection limit was 1.0 mg/kg, and the quantitative limit was 4.0 mg/kg. The results of this research indicate that this method is suitable for the simultaneous determination of glucosamine and galactosamine in food.