Characterization of chemical constituents with their antioxidant and anti-melanogenesis activities from the roots of Ampelopsis japonica. Issue 12 (15th June 2022) - Lee, Chia-Lin
Abstract: Three glycosylated stilbenes (1 –3 ), two anthraquinones (4, 5 ), one lignan (6 ), five tannins (7 –11 ), two amino acids (12, 13 ), and one auronol (14 ) were isolated from the root of Ampelopsis japonica . All compounds, except for 4, 6, and 11 were obtained from this species for the first time. Compounds 6 –9 could notably inhibit ROS generations in HaCaT keratinocyte cells with IC50 values of 5.28, 4.83, 0.87, and 1.66 μM, respectively. Compounds 8 –10 showed potent DPPH free radical scavenging effects with IC50 values of 14.37, 16.08, and 12.11 μM, individually. In anti-melanogenesis assay, only 8 and 9 could decrease 7.93% and 11.66% melanin contents induced by α-MSH in B16F10 melanoma cells at 40 μM and moderately inhibit tyrosinase activities. By far, galloylhamameloses 8 and 9 were found to exhibit both antioxidant and anti-melanogenesis properties that could be further developed as cosmeceutical agents for skin disorders. Graphical Abstract: UF0001