FAIM regulates autophagy through glutaminolysis in lung adenocarcinoma. Issue 6 (3rd June 2022)

• Record Type: Journal Article
• Authors: Han, Tianyu; Wang, Pengcheng; Wang, Yanan; Xun, Wenze; Lei, Jiapeng; Wang, Tao; Lu, Zhuo; Gan, Mingxi; Zhang, Wei; Yu, Bentong; Wang, Jian-Bin
• Is Part Of: Autophagy. Volume 18:Issue 6(2022)
• Page Start: 1416
• Page End: 1432
• Publication Date: 2022-06-03
• Subjects: Autophagy -- Fas apoptosis inhibitory molecule 1 -- glutaminase C -- protein stability -- tetramer formation; Autophagic vacuoles -- Periodicals; Apoptosis -- Periodicals; Cell death -- Periodicals; Lysosomes -- Periodicals; Degeneration (Pathology) -- Periodicals; Autophagy -- Periodicals; Cell Death -- Periodicals; Lysosomes -- Periodicals; Periodicals; 571.936
• DOI: 10.1080/15548627.2021.1987672
• Languages: English
• ISSNs: 1554-8627

Abstract

ABSTRACT: Altered glutamine metabolism is an important aspect of cancer metabolic reprogramming. The GLS isoform GAC (glutaminase C), the rate-limiting enzyme in glutaminolysis, plays a vital role in cancer initiation and progression. Our previous studies demonstrated that phosphorylation of GAC was essential for its high enzymatic activity. However, the molecular mechanisms for GAC in maintaining its high enzymatic activity and protein stability still need to be further clarified. FAIM/FAIM1 (Fas apoptotic inhibitory molecule) is known as an important anti-apoptotic protein, but little is known about its function in tumorigenesis. Here, we found that knocking down FAIM induced macroautophagy/autophagy through suppressing the activation of the MTOR pathway in lung adenocarcinoma. Further studies demonstrated that FAIM could promote the tetramer formation of GAC through increasing PRKCE/PKCε-mediated phosphorylation. What's more, FAIM also stabilized GAC through sequestering GAC from degradation by protease ClpXP. These effects increased the production of α-ketoglutarate, leading to the activation of MTOR. Besides, FAIM also promoted the association of ULK1 and MTOR and this further suppressed autophagy induction. These findings discovered new functions of FAIM and elucidated an important molecular mechanism for GAC in maintaining its high enzymatic activity and protein stability.