Challenges for the determination of spiramycin in aqueous matrices using LC-MS/MS: evidence for the solvent intrusion on the molecule integrity. Issue 27 (9th June 2022)

• Record Type: Journal Article
• Authors: Nasiri, Azadeh; Mokhtari, Shaya; Jahani, Reza; Daraie, Bahram; Yazdanpanah, Hassan; Faizi, Mehrdad; Kobarfard, Farzad
• Is Part Of: RSC advances. Volume 12:Issue 27(2022)
• Page Start: 17096
• Page End: 17103
• Publication Date: 2022-06-09
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra00205a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Water, ethanol, and methanol as protic solvents can add to the formyl group of spiramycin molecules during standard solutions preparation while there was no evidence for the addition of acetonitrile and dimethyl sulfoxide as aprotic solvents. Abstract : Liquid chromatography-tandem mass spectroscopy (LC-MS/MS) is an accurate and specific technique for drug residue analysis in different matrices. The high specificity and sensitivity of the multiple reaction monitoring (MRM) approach for detecting drugs such as aldehydes, which have the potential to change mass during the sample preparation phase, becomes a drawback during the analysis process. In this study, concerns about the intrusion of solvent molecules into spiramycin's chemical structure as an aldehydic drug as well as the stability of spiramycin in the milk matrix were addressed. Furthermore, the binding sites where the solvent molecules could bind to spiramycin molecules were investigated through nuclear magnetic resonance (NMR) spectroscopy. It was revealed that water, ethanol, and methanol as protic solvents can add to the formyl group of spiramycin molecules during standard solutions preparation while there was no evidence for the addition of acetonitrile and dimethyl sulfoxide (aprotic solvents). In addition, as time passed, the peak area of spiramycin decreased either in the spiked aqueous sample or milk sample while an increase in the peak area of H2 O-bound spiramycin was observed. After 96 h, more than 90% of spiramycin was converted to H2 O-bound spiramycin. In conclusion, we can propose the use of aprotic solvents for the preparation of spiramycin standard solutions especially when the prepared solutions are not used instantly. Moreover, ion transitions for both spiramycin and its H2 O-added form (843.6 m / z to 173.9 m / z and 861.5 m / z to 173.9 m / z, respectively) should be considered for the accurate quantification of spiramycin residue in aqueous samples such as milk.