Analyses on intracellular Fe3+ with a rhodamine probe: "turn-on" response, specific recognition and bioimaging. Issue 23 (27th May 2022)

• Record Type: Journal Article
• Authors: Cheng, Zhao; Liu, Xiaojing; Zheng, Lei; Xu, Yue; He, Hao
• Is Part Of: Analytical methods. Volume 14:Issue 23(2022)
• Page Start: 2277
• Page End: 2283
• Publication Date: 2022-05-27
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d2ay00280a
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : The specific recognition and localization of intracellular Fe 3+ by a rhodamine probe. Abstract : To overcome the existing difficulty in distinguishing ferric from ferrous ions, a rhodamine-containing probe was designed to combine with Fe 3+ based on an opening–closing transformation of the spirolactam ring in the rhodamine moiety. Through a specific and stoichiometric fluorescence response towards Fe 3+ by 1 : 1 binding accompanied by an obvious color change in the recognition process of Fe 3+, a "naked-eye" detection method of Fe 3+ in an aqueous environment is possible. Theoretical calculations gave a possible recognition mechanism of the probe–Fe 3+ system. Further cytotoxicity and bioimaging in living L929 cells suggested the probe's future applications as a real-time analytical method for intracellular Fe 3+ in clinical diagnosis. Besides, bioimaging applications enable the dynamic labelling and tracking of Fe 3+ in biological systems.