Physicochemical properties, immunostimulatory and antioxidant activities of a novel polysaccharide isolated from Mirabilis himalaica (Edgew) Heim. Issue 27 (10th June 2022)

• Record Type: Journal Article
• Authors: Bo, Surina; Dan, Mu; Han, Wenjie; Ochir, Sarangua; Bao, Liang; Liu, Lingwei; Muschin, Tegshi; Baigude, Huricha
• Is Part Of: RSC advances. Volume 12:Issue 27(2022)
• Page Start: 17264
• Page End: 17275
• Publication Date: 2022-06-10
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra00060a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Herbal medicines often contain bioactive polysaccharides. Abstract : Herbal medicines often contain bioactive polysaccharides. However, many medicinal herbs have not been explored for any active saccharides that may play key roles in their bioactivities. Herein, we extracted a novel polysaccharide from Mirabilis himalaica (Edgew) heim (denoted MHHP), a popular medicinal ingredient in traditional medicines. The structural and morphological characteristics of MHHP were measured and elucidated by high-performance gel permeation chromatography, gas chromatography connected with mass spectrometry, Fourier transform infrared and nuclear magnetic resonance spectroscopy as well as scanning electron microscopy. MHHP was homogeneous with a molecular weight of 16.1 kDa, M w / M n = 1.33, containing mainly α-d -glucan residues with (1→4)-linkage. The biological activities of MHHP upon proliferation of splenic lymphocyte, activation of related cytokine and production of nitric oxide (NO) in RAW264.7 cells were investigated in vitro . MHHP induced proliferation of mouse spleen lymphocytes and significantly promoted the secretion in TNF-α, IL-6 and NO production in RAW264.7 cells. Meanwhile, MHHP exhibited relatively low antioxidant abilities. Our data suggested that MHHP may have potential immunoregulatory and anti-inflammatory activity, with a moderate antioxidant activity.