Synthesis of bio-templated clickable quantum dots and a dual-emitting organic/inorganic complex for ratiometric fluorescence visual assay of blood glucose. Issue 23 (25th May 2022)

• Record Type: Journal Article
• Authors: Yang, Yeling; Mao, Guobin; Ji, Xinghu; He, Zhike
• Is Part Of: Journal of materials chemistry. Volume 10:Issue 23(2022)
• Page Start: 4473
• Page End: 4478
• Publication Date: 2022-05-25
• Subjects: Materials -- Periodicals; Chemistry, Analytic -- Periodicals; Biomedical materials -- Research -- Periodicals; 543.0284
• DOI: 10.1039/d2tb00435f
• Languages: English
• ISSNs: 2050-750X

Abstract

Abstract : We report the fabrication of a dual-emitting organic/inorganic complex, which was constructed by DBCO/FAM-GOx and bio-templated N3 –QDs. The DNA-bridging FAM@GOx–QDs complex could be applied for the differentiation of diabetic and healthy people by the naked eye. Abstract : With the prevalence of diabetes, rapid and simple blood glucose monitoring has become more and more important. Here, we report the synthesis of bio-templated N3 –CdZnTeS quantum dots (QDs), which are great fluorescent biological labels and were used for the fabrication of dual-emissive dye@protein–QD conjugates via copper-free click chemistry, such as the 5(6)-carboxyfluorescein@glucose oxidase-quantum dot (FAM@GOx–QDs) complex. When adding glucose, the red fluorescence of the CdZnTeS QDs sharply decreased, while the green fluorescence of FAM was invariable. A good linear relationship ranging from 0.3 to 30 μM was obtained for glucose detection, with the limit of detection as low as 0.035 μM. Notably, the DNA-bridging FAM@GOx–QDs complex exhibited enhanced enzyme activity and stability, and was applied for the differentiation of diabetic and healthy people by the naked eye.