Ammonia removal through combined methane oxidation and nitrification-denitrification and the interactions among functional microorganisms. (1st January 2021)

• Record Type: Journal Article
• Authors: Cao, Qin; Li, Xiaochuan; Jiang, Huier; Wu, Han; Xie, Zhijie; Zhang, Xiaoyi; Li, Na; Huang, Xinyi; Li, Zhidong; Liu, Xiaofeng; Li, Dong
• Is Part Of: Water research. Volume 188(2021)
• Publication Date: 2021-01-01
• Subjects: Methane oxidation -- Heterotrophic nitrification-aerobic denitrification -- Coupling mechanism -- Microbial diversity; AME-D methane oxidation coupled to denitrification -- ME-SND methane oxidation coupled to simultaneous nitrification and denitrification -- HN-AD heterotrophic nitrification and aerobic denitrification -- HfMBR hollow-fiber membrane biofilm reactor -- AME-D3 methane oxidation coupled to nitrate denitrification -- AME-D2 methane oxidation coupled to nitrite denitrification -- AOB ammonia-oxidizing bacteria -- NOB nitrite-oxidizing bacteria -- pmoA particulate methane monooxygenase -- nirS cytochrome cd1 nitrite reductase -- AOB-amoA ammonia monooxygenase in ammonia-oxidizing bacteria -- A/O anoxic/Oxic -- A2/O anaerobic-anoxic-Oxic -- COD chemical oxygen demand -- TIN total inorganic nitrogen -- TN total nitrogen; Water -- Pollution -- Research -- Periodicals; 363.7394
• DOI: 10.1016/j.watres.2020.116555
• Languages: English
• ISSNs: 0043-1354

Abstract

Highlights: NO3 − and NO2 − denitrification were confirmed to be coupled to CH4 oxidation. The nitrogen removal rates were highest in 25% O2 in AME-D3 and 20% O2 in AME-D2. In ME-SND, the highest NH4 + -N removal rate reached 22.03 mg/(Ld) in group 25% O2 . The primary methanotrophs in ME-SND were Methylobacter and Methylomonas. The main HN-AD bacteria in ME-SND were Chryseobacterium and Comamonas . Abstract: It would be highly beneficial to use the methane produced by anaerobic digestion, which is low cost and accessible, as the carbon source in the removal of nitrogenous contaminants in wastewater. However, there is a knowledge gap regarding coupling systems that entail methane oxidation, nitrification, and denitrification, which restricts their industrial application. In this study, we acclimated a mixed culture to deal with simultaneous nitrification-denitrification coupled to methane oxidation in a laboratory-scale hollow-fiber membrane biofilm reactor, which achieved a steady ammonia removal rate of 38.09 mg N/(Ld). Furthermore, a series of batch experiments were conducted to test methane oxidation coupled to nitrate denitrification (AME-D3), nitrite denitrification (AME-D2), and simultaneous nitrification and denitrification (ME-SND). The molar ratio between methane consumed and nitrate reduced (C/N) equals 10 and 5 mol CH4 C mol −1 NO3 N in AME-D3 and AME-D2, averagely and respectively. Without methane injection, the removal of nitrates and nitrites was very low, indicating that the coupling of nitrate/nitrite denitrification and methane oxidation was beneficial. The average ammonia removal rates in the 20% O2 and 25% O2 groups were 20.06 and 22.03 mg N/(Ld) in the ME-SND system, respectively. Without methane, the ammonia oxidation rate declined, and large amounts of nitrite accumulated. As traditional ammonia and nitrite oxidation approaches are autotrophic, we proposed the possibility of heterotrophic nitrification-aerobic denitrification (HN-AD). To study the coupling systems, the microbial communities and functional bacteria were analyzed. The results indicated that the system contained a guild of methanotrophs (mainly Methylobacter ) and HN-AD bacteria (mainly Chrysobacterium and Comamonas ). Graphical abstract: Image, graphical abstract