Enhancement of ADP release from the RAD51 presynaptic filament by the SWI5-SFR1 complex. Issue 1 (27th September 2013)

• Record Type: Journal Article
• Authors: Su, Guan-Chin; Chung, Chan-I; Liao, Chia-Yu; Lin, Sheng-Wei; Tsai, Cheng-Ting; Huang, Tao; Li, Hung-Wen; Chi, Peter
• Is Part Of: Nucleic acids research. Volume 42:Issue 1(2014)
• Page Start: 349
• Page End: 358
• Publication Date: 2013-09-27
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkt879
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: Homologous recombination catalyzed by the RAD51 recombinase eliminates deleterious DNA lesions from the genome. In the presence of ATP, RAD51 forms a nucleoprotein filament on single-stranded DNA, termed the presynaptic filament, to initiate homologous recombination-mediated DNA double-strand break repair. The SWI5-SFR1 complex stabilizes the presynaptic filament and enhances its ability to mediate the homologous DNA pairing reaction. Here we characterize the RAD51 presynaptic filament stabilization function of the SWI5-SFR1 complex using optical tweezers. Biochemical experiments reveal that SWI5-SFR1 enhances ATP hydrolysis by single-stranded DNA-bound RAD51. Importantly, we show that SWI5-SFR1 acts by facilitating the release of ADP from the presynaptic filament. Our results thus provide mechanistic understanding of the function of SWI5-SFR1 in RAD51-mediated DNA recombination.