Inhibition of Steptococcus mutans biofilm formation by extracts of Tenacibaculum sp. 20J, a bacterium with wide-spectrum quorum quenching activity. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Muras, Andrea; Mayer, Celia; Romero, Manuel; Camino, Tamara; Ferrer, Maria D.; Mira, Alex; Otero, Ana
• Is Part Of: Journal of oral microbiology. Volume 10:Issue 1(2018)
• Publication Date: 2018-01-01
• Subjects: Quorum sensing -- quorum quenching -- AI-2 -- biofilms -- dental plaque -- streptococci; Mouth -- Microbiology -- Periodicals; Medical microbiology -- Periodicals; Mouth Diseases -- microbiology; Mouth -- microbiology; Medical microbiology; Mouth -- Microbiology; Oral Pathology; Microbiology; Periodicals; Periodicals; Fulltext; Internet Resources; Periodicals; Electronic journals; 616.31
• DOI: 10.1080/20002297.2018.1429788
• Languages: English
• ISSNs: 2000-2297

Abstract

ABSTRACT: Background : Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective : To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design : The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system®, confocal laser microscopy, and the crystal violet quantification method. Results : The addition of the cell extract from Tenacibaculum sp. 20J reduced biofilm formation in S. mutans ATCC25175 by 40–50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in S. oralis DSM20627 and S. dentisani 7747 biofilms. Conclusions : The ability of Tenacibaculum sp. 20J to interfere with AI-2 and inhibit biofilm formation in S. mutans was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required.