Secretion of proteins and antibody fragments from transiently transfected endothelial progenitor cells. Issue 15 (1st July 2020)

• Record Type: Journal Article
• Authors: Heller, Loree; Thinard, Reynald; Chevalier, Melanie; Arpag, Sezgi; Jing, Yu; Greferath, Ruth; Heller, Richard; Nicolau, Claude
• Is Part Of: Journal of cellular and molecular medicine. Volume 24:Issue 15(2020)
• Page Start: 8772
• Page End: 8778
• Publication Date: 2020-07-01
• Subjects: electroporation -- endothelial progenitor cells -- ex vivo cell therapy -- gene therapy -- β‐amyloid disaggregation; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.15511
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood‐brain barrier (BBB) breakdown. After intravenous or intra‐arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to promote neurovascular repair. Autologous EPCs transfected to express specific therapeutic proteins offer an innovative therapeutic option. Here, we demonstrate that EPC transfection by electroporation with plasmids encoding the reporter protein GFP or an anti‐β‐amyloid antibody fragment (Fab) leads to secretion of each protein. We also demonstrate the secreted anti‐β‐amyloid Fab protein functions in β‐amyloid aggregate solubilization.