Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S‐sulfhydration. Issue 16 (7th July 2020)

• Record Type: Journal Article
• Authors: Lu, Fangping; Lu, Baoling; Zhang, Linxue; Wen, JingChen; Wang, Mengyi; Zhang, Shiwu; Li, Qianzhu; Shu, Feng; Sun, Yu; Liu, Ning; Peng, Shuo; Zhao, Yajun; Dong, Shiyun; Zhao, Dechao; Lu, Fanghao; Zhang, Weihua
• Is Part Of: Journal of cellular and molecular medicine. Volume 24:Issue 16(2020)
• Page Start: 9362
• Page End: 9377
• Publication Date: 2020-07-07
• Subjects: diabetes mellitus -- hydrogen sulphide -- Muscle RING finger 1 -- skeletal muscle atrophy -- S‐sulfhydration modification; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.15587
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Muscle atrophy occurs in many pathological states, including cancer, diabetes and sepsis, whose results primarily from accelerated protein degradation and activation of the ubiquitin‐proteasome pathway. Expression of Muscle RING finger 1 (MuRF1), an E3 ubiquitin ligase, was increased to induce the loss of muscle mass in diabetic condition. However, hydrogen sulphide (H2 S) plays a crucial role in the variety of physiological functions, including antihypertension, antiproliferation and antioxidant. In this study, db/db mice and C2C12 myoblasts treated by high glucose and palmitate and oleate were chose as animal and cellular models. We explored how exogenous H2 S attenuated the degradation of skeletal muscle via the modification of MuRF1 S‐sulfhydration in db/db mice. Our results show cystathionine‐r‐lyase expression, and H2 S level in skeletal muscle of db/db mice was reduced. Simultaneously, exogenous H2 S could alleviate ROS production and reverse expression of ER stress protein markers. Exogenous H2 S could decrease the ubiquitination level of MYOM1 and MYH4 in db/db mice. In addition, exogenous H2 S reduced the interaction between MuRF1 with MYOM1 and MYH4 via MuRF1 S‐sulfhydration. Based on these results, we establish that H2 S prevented the degradation of skeletal muscle via MuRF1 S‐sulfhydration at the site of Cys44 in db/db mice.