Allyl isothiocyanate induces stomatal closure in Vicia faba. Issue 10 (3rd October 2015)

• Record Type: Journal Article
• Authors: Sobahan, Muhammad Abdus; Akter, Nasima; Okuma, Eiji; Uraji, Misugi; Ye, Wenxiu; Mori, Izumi C; Nakamura, Yoshimasa; Murata, Yoshiyuki
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 79:Issue 10(2015)
• Page Start: 1737
• Page End: 1742
• Publication Date: 2015-10-03
• Subjects: guard cells -- peroxidase -- signaling -- stomatal closure; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2015.1045827
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Isothiocyanates are enzymatically produced from glucosinolates in plants, and allyl isothiocyanate (AITC) induces stomatal closure in Arabidopsis thaliana . In this study, we investigated stomatal responses to AITC in Vicia faba . AITC-induced stomatal closure accompanied by reactive oxygen species (ROS) and NO production, cytosolic alkalization and glutathione (GSH) depletion in V. faba . GSH monoethyl ester induced stomatal reopening and suppressed AITC-induced GSH depletion in guard cells. Exogenous catalase and a peroxidase inhibitor, salicylhydroxamic acid, inhibited AITC-induced stomatal closure, unlike an NAD(P)H oxidase inhibitor, diphenylene iodonium chloride. The peroxidase inhibitor also abolished the AITC-induced ROS production, NO production, and cytosolic alkalization. AITC-induced stomatal closure was suppressed by an NO scavenger, 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide, and an agent to acidify cytosol, butyrate. These results indicate that AITC-induced stomatal closure in V. faba as well as in A. thaliana and suggest that AITC signaling in guard cells is conserved in both plants. Graphical abstract: : Signal transduction of allyl isothiocyanate in Vicia faba guard cells .