MiR-10b-5p Regulates C2C12 Myoblasts Proliferation and Differentiation. Issue 2 (1st February 2019)

• Record Type: Journal Article
• Authors: Ge, Guihua; Yang, Dongli; Tan, Ya; Chen, Ying; Jiang, Dongmei; Jiang, Anan; Li, Qiang; Liu, Yihui; Zhong, Zhijun; Li, Xuewei; Zhang, Shunhua; Zhu, Li
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 2(2019)
• Page Start: 291
• Page End: 299
• Publication Date: 2019-02-01
• Subjects: Mir-10b-5p -- NFAT5 -- myogenesis -- C2C12 myoblasts; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1533805
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: The development of skeletal muscle is a complex process including myoblasts proliferation and differentiation. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at post-transcriptional level. Increasing evidences indicate that miRNAs are important regulators in myogenic processes. Here, we reported that the expression of miR-10b-5p steadily decreased during myoblasts proliferation, but significantly increased during myoblasts differentiation. The over-expression of miR-10b-5p promoted myoblasts proliferation and blunted myofiber formation in C2C12 cells, while miR-10b-5p down-regulation showed an opposite result. At the same time, we observed that the down-regulation of nuclear factor of activated T-cells 5 ( NFAT5 ) repressed the differentiation of C2C12 cells, and interestingly, miR-10b-5p could suppress NFAT5 expression. Luciferase activity assays confirmed that miR-10b-5p directly target the 3ʹ-untranslated region (3ʹ-UTR) of NFAT5 . Overall, we proposed here a novel insight that miR-10b-5p regulates the proliferation and differentiation of C2C12 myoblasts, and the impact on myogenic differentiation is partly through targeting NFAT5 . Abbreviations : NFAT5: nuclear factor of activated T-cells 5; Cyclin B: cycle protein B; Cyclin D1: cycle protein D1; Cyclin E: cycle protein E; CDK4: cyclin-dependent kinase 4; MyoD: myogenic differentiation antigen; MyoG: myogenin; Myf5: myogenic factor 5; MRF4: myogenic regulatory factor 4; MyHC: myosin heavy chain; AQP5: aquaporin-5; CACNA1C: calcium voltage-gated channel subunit alpha1 C; SRF: serum response factor; Pax7: paired box 7; KLF4: Kruppel-like factor 4; 3'-UTR: 3'-untranslated region; GM: growth medium; DM: differentiation medium Graphical abstract: : miR-10b-5p promoted myoblasts proliferation but repressed differentiation. The impact on myogenic differentiation is partly through targeting NFAT5.