Localization of the CAPRICE-ENHANCER OF TRY AND CPC1 chimera protein in Arabidopsis root epidermis. Issue 9 (2nd September 2017)

• Record Type: Journal Article
• Authors: Tominaga-Wada, Rumi; Kurata, Tetsuya; Wada, Takuji
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 9(2017)
• Page Start: 1762
• Page End: 1767
• Publication Date: 2017-09-02
• Subjects: Arabidopsis -- CPC -- ETC1 -- root hair -- trichome; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1343120
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: The CAPRICE ( CPC ) encodes an R3-type MYB transcription factor, which promotes root-hair differentiation. Previously, we showed that the CPC protein moves from the non-hair cell to the neighboring cell and induces root-hair differentiation in Arabidopsis . In addition, we proposed two cell-to-cell movement signal sequences, S1 and S2, in CPC. However, an S1:2xGFP:S2 chimera protein did not move between root epidermal cells. Here, we show that the S1 and S2 sequences do not confer cell-to-cell movement or nuclear localization ability to a GFP protein. The ENHANCER OF TRY AND CPC1 ( ETC1 ) gene encodes the CPC homolog R3 MYB; this protein does not possess cell-to-cell movement ability or the S1 sequence. To elucidate whether the S1 sequence can induce cell-to-cell movement ability in ETC1, CPCp:S1:ETC1:2xGFP was constructed and introduced into Arabidopsis . Our results indicate that the addition of the S1 sequence was not sufficient for ETC1 to acquire cell-to-cell movement ability. Abstract : :