Polysaccharide hydrolase of the hadal zone amphipods Hirondellea gigas. Issue 7 (3rd July 2018)

• Record Type: Journal Article
• Authors: Kobayashi, Hideki; Nagahama, Takahiko; Arai, Wataru; Sasagawa, Yohei; Umeda, Mana; Hayashi, Tetsutaro; Nikaido, Itoshi; Watanabe, Hiromi; Oguri, Kazumasa; Kitazato, Hiroshi; Fujioka, Kantaro; Kido, Yukari; Takami, Hideto
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 82:Issue 7(2018)
• Page Start: 1123
• Page End: 1133
• Publication Date: 2018-07-03
• Subjects: Hirondellea gigas -- hadal zone -- polysaccharide hydrolase -- cellulose -- amphipod; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1459178
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: Hirondellea species are common inhabitants in the hadal region deeper than 7, 000 m. We found that Hirondellea gigas thrived in the Challenger Deep possessed polysaccharide hydrolases as digestive enzymes. To obtain various enzymes of other H. gigas, we captured amphipods from the Japan Trench, and Izu-Ogasawara (Bonin) Trench. A phylogenetic analysis based on the cytochrome oxidase I gene showed close relationships among amphipods, despite the geographic distance between the localities. However, several differences in enzymatic properties were observed in these H. gigas specimens. We also carried out RNA sequencing of H. gigas from the Izu-Ogasawara Trench. The cellulase gene of H. gigas was highly homologous to cellobiohydrolase of Glucosyl Hydrolase family 7 (GH7). On the other hand, enzymatic properties of H. gigas 's cellulase were different from those of typical GH7 cellobiohydrolase. Thus, these results indicate that hadal-zone amphipod can be good candidates as the new enzyme resource. Abstract: : The deepest sea amphipod, Hirondellea gigas digests plant debris with GH7 cellobiohydrolase, GH9 ß-1. 4 glucanase, and amylase to obtain nutrients in oligotrophic sea bottom.