Effect of mutation of C-terminal and heme binding region of Arabidopsis catalase on the import to peroxisomes. Issue 2 (1st February 2019)

• Record Type: Journal Article
• Authors: Fujikawa, Yukichi; Suekawa, Marina; Endo, Satoshi; Fukami, Youjirou; Mano, Shoji; Nishimura, Mikio; Esaka, Muneharu
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 83:Issue 2(2019)
• Page Start: 322
• Page End: 325
• Publication Date: 2019-02-01
• Subjects: Catalase -- Heme-binding -- Peroxisomal protein import -- Peroxisome targeting signal; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2018.1530094
• Languages: English
• ISSNs: 0916-8451

Abstract

ABSTRACT: We evaluated the import of Arabidopsis catalase to peroxisomes under homogenous transient expression. The amino acids at −11 to −4 from the C-terminus are necessary for catalase import. The results are in agreement with the previous work under stable expression. We first demonstrate that heme-binding sites are important for peroxisomal import, suggesting the importance of catalase folding. Abbreviations : AtCat: Arabidopsis catalase; PTS: peroxisomal targeting signal; PEX: Peroxin