3, 4-Dihydroxyphenylacetic acid is a potential aldehyde dehydrogenase inducer in murine hepatoma Hepa1c1c7 cells. Issue 10 (3rd October 2017)

• Record Type: Journal Article
• Authors: Liu, Yujia; Kurita, Ayuki; Nakashima, Sayaka; Zhu, Beiwei; Munemasa, Shintaro; Nakamura, Toshiyuki; Murata, Yoshiyuki; Nakamura, Yoshimasa
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 10(2017)
• Page Start: 1978
• Page End: 1983
• Publication Date: 2017-10-03
• Subjects: 3, 4-dihydroxyphenylacetic acid -- aldehyde dehydrogenase -- acetaldehyde -- Nrf2 -- Hepa1c1c7 cells; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1361809
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: 3, 4-Dihydroxyphenylacetic acid (DOPAC) is one of the major colonic microflora-produced catabolites of quercetin glycosides, such as quercetin 4′-glucoside derived from onion. Here, we investigated whether DOPAC modulates the aldehyde dehydrogenase (ALDH) activity and protects the cells from the acetaldehyde-induced cytotoxicity in vitro . DOPAC was shown to enhance not only the total ALDH activity, but also the gene expression of ALDH1A1, ALDH2 and ALDH3A1 in a concentration-dependent manner. DOPAC simultaneously stimulated the nuclear translocation of NFE2-related factor 2 and aryl hydrocarbon receptor. The pretreatment of DOPAC completely protected the cells from the acetaldehyde-induced cytotoxicity. The present study suggested that DOPAC acts as a potential ALDH inducer to prevent the alcohol-induced abnormal reaction. Abstract: : 3, 4-Dihydroxyphenylacetic acid, a colonic microflora-produced catabolite of quercetin glycosides, protects the cells from the acetaldehyde-induced cytotoxicity.