Tripterygium regelii decreases the biosynthesis of triacylglycerol and cholesterol in HepG2 cells. Issue 12 (2nd December 2017)

• Record Type: Journal Article
• Authors: Kang, Myung-Ji; Kwon, Eun-Bin; Yuk, Heung Joo; Ryu, Hyung Won; Kim, Soo-Yeon; Lee, Mi-Kyeong; Moon, Dong-Oh; Lee, Su Ui; Oh, Sei-Ryang; Lee, Hyun-Sun; Kim, Mun-Ock
• Is Part Of: Bioscience, biotechnology, and biochemistry. Volume 81:Issue 12(2017)
• Page Start: 2285
• Page End: 2291
• Publication Date: 2017-12-02
• Subjects: Tripterygium regelii -- triacylglycerol -- cholesterol -- SREBP1c -- SREBP2; Biotechnology -- Periodicals; Biochemistry -- Periodicals; 660.6
• DOI: 10.1080/09168451.2017.1390392
• Languages: English
• ISSNs: 0916-8451

Abstract

Abstract: In the course of screening to find a plant material decreasing the activity of triacylglycerol and cholesterol, we identified Tripterygium regelii (TR). The methanol extract of TR leaves (TR-LM) was shown to reduce the intracellular lipid contents consisting of triacylglycerol (TG) and cholesterol in HepG2 cells. TR-LM also downregulated the mRNA and protein expression of the lipogenic genes such as SREBP-1 and its target enzymes. Consequently, TR-LM reduced the TG biosynthesis in HepG2 cells. In addition, TR-LM decreased SREBP2 and its target enzyme HMG-CoA reductase, which is involved in cholesterol synthesis. In this study, we evaluated that TR-LM attenuated cellular lipid contents through the suppression of de novo TG and cholesterol biosynthesis in HepG2 cells. All these taken together, TR-LM could be beneficial in regulating lipid metabolism and useful preventing the hyperlipidemia and its complications, in that liver is a crucial tissue for the secretion of serum lipids. Abstract: : The methanol extract of Tripterygium regelii leaves reduced intracellular contents of TG and cholesterol in HepG2 cells.